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First name*

Last name*

Email*

Desired resolution*

Catalog Number*

Lot Number*

Order Number*

How many times was the experiment repeated?*

Were the outcomes the same every time?*

How do the observed results differ from the expected results?*

How were the products stored?*

According to the datasheet

other

Which samples did you use?*

Serum

Plasma

Tissue Homogenate

Biological Fluids (Urine etc.)

Cell Culture Supernatant

Protein (recombinant, purified)

Other

How long were the samples stored?*

What conditions were the samples stored in?*

What groups of samples did you use?*

Normal Group

Model Group

Patients Group

Other

Which sample species did you use?*

Human

Mouse

Rat

Other

Which lysis buffer did you use? *

Kit Supplied Lysis Buffer

RIPA Buffer

SDS Buffer

Other

Did you sonicate the samples?*

Yes

What was the sample dilution used?*

1:100

1:50

1:10

No dilution

Other

How did you dilute your samples? Please explain if you used standard diluent, PBS, etc.*

10 µl Samples are diluted with 90µl of Sample Diluent

How much sample (lysate) was loaded per well? *

100ul

20ul

Other

11. Did you use negative and/or positive controls? If yes, please indicate the type.*

Postive Control

Negative Control

No Controls

Protocol Details

12. Were all reagents and samples brought to room temperature before use? *

Yes

How did you perform the standard dilution? *

According to the datasheet

Other

Did you perform any steps differently than stated in the datasheet?

Experimental Results

Please include any additional details here:

Required Test Data

Plate design/ distribution of samples, controls and standards
Standard curve (including test results with OD and standard concentration)
Sample data (please provide the data in Excel table format)
% CV values (if you encountered high variation / %CV values please indicate the replicates and state the CV values calculated)

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